Think of a 24-hour-a-day seven-day-a-week radio station playing a style of music you like e.g. pop, rock, country etc.

Once you've done the work to tune your radio to that station ("you changed your vibrational setpoint on a subject to something good-feeling"), the radio will continue to play music from that station every time you turn it on.

Even if you turn it off ("fall out of alignment with your Broader Self"), the radio will remain tuned in to the station ready for when you decide to turn it on again ("start feeling good again").

And it doesn't matter how many times you turn your radio on and off because the reception frequency is set and the station is always broadcasting.

As with all radio stations, sometimes the actual songs that are played are ones you love ("you feel really inspired to do something") and sometimes you don't like them ("you don't feel inspired").

If the station plays a song you don't like ("you don't feel inspired"), you never need to worry because, sooner or later, they will play a song you do like again ("you get another burst of inspiration") because you know you like the general style of music this station plays.

My main question is if you will get any desire even if you miss inspiration multiple times

There are really only two ways for you to interfere with hearing songs you like from that radio station.

The first is to keep your radio permanently turned off ("keep yourself generally out of alignment with your broader self i.e. out of the Vortex").

The second is to retune your radio permanently to another station that plays a different style of music you don't like ("change your vibrational setpoint regarding the subject to something bad-feeling").

But if you think about it, even if you haven't got your radio tuned to that particular station you like ("you haven't changed a particular vibrational setpoint to a good-feeling one"), as long as you keep your radio turned on ("keep generally in alignment") you are still increasing the chances of eventually hearing something you do like even if it is being broadcast by another station playing a different style of music.

Summary

As long as you stay generally good feeling, you'll still be getting inspirations and opportunities from time to time that will lead you to what you want even if you are not aligned on your "target" subject.

The opportunities will keep on coming forever as long as you stay in a generally good feeling place (i.e "the radio is kept turned on").

As a bonus, if you are also aligned on your "target" subject - i.e. you've done the vibrational work to feel good about the subject - then it greatly increases the opportunities and inspirations that will come your way and you won't have to wait as long for them.

It's probably not a perfect analogy (none ever are), but hopefully it gives you a feel for how these things work :)

ADDED - MAY 11, 2015

In response to a question posed by @Mariposa on this thread...

Hello. I have read on here before that the best way to manifest is to think about it once and totally give up on it ever coming. Now here it says that you have a better chance to manifest if you keep aligning yourself positively with your desire. This seem contradictory to me.

Most people define "best" as meaning the maximum results for minimum effort.

So, in that sense, the "best" manifesting approach is to think very clearly about what you want (from a good-feeling place) and never think about it again i.e. let it go completely.

Sure, that will absolutely get you what you want. (See Manifesting Experiment 1)

The small teeny-weeny tricky aspect to this :) is that most people don't try out these ideas with subjects they can forget about easily. They will usually try out these ideas with the biggest, baddest, toughest, hardest things in their life first :)

It's like taking your first guitar lesson ever and then immediately going on stage in a huge arena full of thousands of screaming fans demanding the best solo guitar performance they've ever heard in their lives...well...err...someone's going to end up disappointed :)

Even the slightest re-peeking at your desire can activate enough lack to stop it manifesting physically.

So if you can't forget about what you want completely, the next best thing is to always feel good when you think about it - and so you need to change your vibrational setpoint regarding that subject so it always feels good if you ever happen to think about it again.

That's kind of what the first part of this answer (above) is getting at.

It doesn't mean you won't get your desire if you don't always feel good about it because there is a Reach Down From The Vortex effect also at work if you keep yourself generally aligned.

Having said all of that, even if you could completely forget about your desire, it may be of more value to you to not actually forget about it :)

This is the bit that people really hate to hear :) ...the problem is that in order to be vibrationally lined-up enough to get your desire, you must have neutralized most, if not all, of the thrill of wanting it.

So, by the time you are aligned enough to get your desire, it's really No Big Deal that is there and you are already looking for the next thing.

So then the only real pleasure you will get from your desires is before they come when you are still feeling/visualizing the thrill of what it would be like to have them.

So even if you could completely forget about what you want, you probably don't want to because you are depriving yourself of all the good-feeling juice you could squeeze out of the idea of having it.

Hope that answers your question :)